qPCR
نویسندگان
چکیده
منابع مشابه
qPCR 2005 2 nd International qPCR Symposium Industrial Exhibition TATAA
1: In recent years, capillary-based LightCycler® Systems have become an established standard for rapid and accurate real-time PCR. Based on innovative technology for heating/cooling and highly accurate analysis algorithms, they enable thermal cycling at high speed and with precisely controlled temperatures, characteristics directly related to the consistency and accuracy of scientific results. ...
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Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets. Despite the f...
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Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends. When removal of inhibitory substances by column or reagent-based methods fails or is incomplete, t...
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A qPCR array was designed to facilitate forensic serological identification of unknown stains and fluids. In our approach, the flow-through of a DNA purification column is further purified for total RNA. Therefore, the DNA preparation is fully available for forensic DNA analysis whereas the RNA fraction is processed for serological examination by mRNA analysis. The RNA fraction is first subject...
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Scientific conferences fulfil many roles, but one of the most mportant ones is that they help shape the direction in which a scintific discipline grows by promoting person-to-person exchanges f information, ideas and constructive criticisms between scientists rom different backgrounds. This interaction also helps to idenify areas of controversy and promotes efforts to address and, it is oped, r...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113234